Immunization with recombinant E^rns-LTB fusion protein elicits protective immune responses against bovine viral...

Bovine viral diarrhea virus (BVDV), a major infectious pathogen and is associated with major economic losses and significant impact on animal welfare worldwide. Here, recombinant E^rns-LTB protein vaccine containing MF59 adjuvant was prepared and assessed using a mouse model. The recombinant plasmid (pET32a-E^rns-LTB) was constructed and transformed into BL21 (DE3) cells to produce E^rns-LTB protein. The E^rns-LTB protein was formulated with MF59 adjuvant, when delivered intraperitoneally in mice, exhibited higher immunogenic and induced superior levels of anti-BVDV IgG compared with the MF59 adjuvanted E^rns protein. Importantly, after challenged with different BVDV BJ175170 and BJ1305 isolate strains, mice inoculated with E^rns-LTB protein displayed alleviated pathological damage and decreased plasma virus shedding compared with mice inoculated with E^rns protein. The enhanced protection from E^rns-LTB protein is mediated by T cell immunity and primarily based on CD4⁺ T helper (Th) and CD8⁺ cytotoxic T lymphocyte (CTL), these results suggest that E^rns-LTB protein has potential to protect against a broad range of BVDV strains thereby providing a novel direction for developing broadly protective vaccines.