Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.2K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Historical development of FINCH from the beginning to single-shot 3D confocal imaging beyond optical resolution [Invited].

Applied optics·2022
Same author

Chromis-1, a Ratiometric Fluorescent Probe Optimized for Two-Photon Microscopy Reveals Dynamic Changes in Labile Zn(II) in Differentiating Oligodendrocytes.

ACS sensors·2018
Same author

High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers.

Nature photonics·2017
Same author

CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography).

Proceedings of SPIE--the International Society for Optical Engineering·2016
Same author

Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy.

Optics express·2014
Same author

In-line FINCH super resolution digital holographic fluorescence microscopy using a high efficiency transmission liquid crystal GRIN lens.

Optics letters·2013

Related Experiment Video

Updated: Nov 1, 2025

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

7.9K

Single shot holographic super-resolution microscopy.

Nisan Siegel, Gary Brooker

    Optics Express
    |June 22, 2021
    PubMed
    Summary
    This summary is machine-generated.

    A new birefringent FINCH holographic lens system offers simple, versatile super-resolution microscopy. This cost-effective method achieves resolution comparable to complex techniques with a single image capture.

    More Related Videos

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.7K
    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    2.9K

    Related Experiment Videos

    Last Updated: Nov 1, 2025

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
    10:01

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

    Published on: September 8, 2017

    7.9K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.7K
    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    2.9K

    Area of Science:

    • Optical Microscopy
    • Super-resolution Imaging
    • Biophysics

    Background:

    • Super-resolution microscopy techniques enhance optical resolution beyond the diffraction limit.
    • Existing methods like SIM and Airyscan are often complex, requiring extensive calibration and specific light sources.
    • There is a need for accessible, versatile super-resolution techniques applicable across various research settings.

    Purpose of the Study:

    • To develop a simple and versatile super-resolution microscopy system.
    • To achieve resolution comparable to or better than existing advanced methods with a single image capture.
    • To increase the accessibility and utility of super-resolution microscopy for all researchers.

    Main Methods:

    • Integration of a birefringent FINCH (Focal Interference Noumenal Contrast Holography) holographic lens system with a standard microscope.
    • Utilized an inexpensive uncooled CMOS camera for image acquisition.
    • The system operates with a single image capture, requiring no prior knowledge of the sample.

    Main Results:

    • Achieved super-resolution imaging with resolution equivalent to or exceeding established methods like SIM and Airyscan.
    • Demonstrated versatility across all objective powers and numerical apertures (NA).
    • The system is solid-state, fast, calibration-free, and works with any light type (fluorescence, bioluminescence, reflected light).

    Conclusions:

    • The new FINCH implementation provides a significant advance in super-resolution microscopy, offering high resolution with exceptional simplicity.
    • Its ease of operation, maintenance, and broad applicability make super-resolution microscopy more accessible to any research laboratory.
    • This technique democratizes advanced imaging capabilities, enabling new discoveries across diverse scientific fields.