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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

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Speckle-dependent accuracy in phase-sensitive optical coherence tomography.

Matt S Hepburn, Ken Y Foo, Philip Wijesinghe

|June 22, 2021

Summary

This summary is machine-generated.

Speckle in phase-sensitive optical coherence tomography (OCT) significantly reduces motion measurement accuracy. A new metric, speckle brightness, quantifies this effect, improving accuracy in optical coherence elastography (OCE).

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Area of Science:

Biomedical Optics
Medical Imaging
Optical Metrology

Background:

Phase-sensitive optical coherence tomography (OCT) measures motion using Doppler OCT and optical coherence elastography (OCE).
Current motion estimation models in OCT are based on single reflectors, which is inaccurate for turbid samples like tissue that exhibit speckle.
Speckle, a phenomenon in OCT imaging, is known to affect image quality but its impact on phase-sensitive motion measurement accuracy is not well understood.

Purpose of the Study:

To investigate the impact of speckle on the accuracy of phase-sensitive OCT for motion measurement.
To develop a new metric, speckle brightness, to quantify the influence of speckle on OCT phase sensitivity.
To improve the accuracy and contrast-to-noise ratio in optical coherence elastography (OCE) by accounting for speckle effects.

Main Methods:

Theoretical modeling and experimental validation of speckle's effect on phase-sensitive OCT.
Introduction and application of a novel metric, speckle brightness, to quantify constructive interference in OCT images.
Implementation of independent speckle realizations in compression OCE to enhance strain sensitivity and contrast-to-noise ratio.

Main Results:

Speckle significantly reduces the accuracy of phase-sensitive OCT, an effect not captured by the signal-to-noise ratio (SNR) alone.
A three-fold decrease in sensitivity was observed between high and low speckle brightness regions at constant OCT SNR.
Incorporating independent speckle realizations in compression OCE led to a ten-fold improvement in strain sensitivity and a five-fold improvement in contrast-to-noise.

Conclusions:

Speckle imposes a fundamental limit on the accuracy of phase-sensitive OCT motion measurements.
Speckle brightness is a critical parameter that must be considered for accurate interpretation of OCT experimental data.
Accounting for speckle effects can significantly enhance the performance of OCT-based techniques like OCE.