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Updated: Nov 1, 2025
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Development of a lateral-flow ICA strip for the detection of colchicine
Shaozhen Li1, Jianping Zhu, Wei Ma
1State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn.
Abstract:
Colchicine (COL), which is extracted from colchicine and papaya, is widely used in medicine. However, COL poisoning causes serious adverse complications including death. Therefore, there is a need to develop a sensitive COL detection method. In this study, we developed a highly sensitive monoclonal antibody 1E4 with a half-maximal inhibitory concentration and linear range of 0.43 ng mL-1 and 0.09-2.16 ng mL-1, respectively. Using 1E4, we developed a lateral-flow immunochromatographic assay (ICA) strip for COL detection. Based on the results, the detection interval was 1-25 ng mL-1 in milk, 2.5-50 ng mL-1 in beef, 1-25 ng mL-1 in edible lily and 2.5-25 ng mL-1 in daylily. The lateral-flow ICA strip can be used as an effective tool for COL detection in food samples on site.
