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MiR-150 suppressed cell viability, invasion and EMT via HMGA2 in oral squamous cell carcinoma
1Department of Stomatology, Yantaishan Hospital, Yantai, China. quydtotr508855@163.com.
Objective:
Oral squamous cell carcinoma (OSCC) accounts for 90% of head and neck cancers, and its 5-year overall survival is very poor. MiR-150 is usually downregulated and acts as tumor suppressor in multiple cancers. The aim of our study is to explore the functions of miR-150 in OSCC.
Patients And Methods:
Expressions of miR-150 and HMGA2 mRNA in OSCC tissues and cells were analyzed by qRT-PCR. Methyl Thiazolyl Tetrazolium (MTT) and transwell assays were conducted to assess the cell viability and invasive abilities. Western blot was conducted to assess the protein levels of epithelial-mesenchymal transition (EMT) markers. Luciferase reporter assay was carried out to verify miR-150 directly binding to HMGA2 in SCC25 cells.
Results:
MiR-150 was low expressed and HMGA2 was highly expressed in OSCC tissues and cells. Downregulation of miR-150 or upregulation of HMGA2 predicted poor prognosis of OSCC patients. MiR-150 overexpression inhibited the abilities of viability, invasive and the EMT by targeting HMGA2 in OSCC cells. HMGA2 was a target gene of miR-150 and its expression was regulated by altering the expression of miR-150 in OSCC cells. HMGA2 reversed partial roles of miR-150 on cell viability and invasion in OSCC.
Conclusions:
MiR-150 impaired cell viability, invasion and EMT via binding to HMGA2 of OSCC. Our research demonstrates that miR-150 plays a critical role in the progression of OSCC. miR-150 might be a candidate molecular marker and a novel therapy target for OSCC patients.
Insights
MicroRNA-150 (miR-150) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). Lower miR-150 levels correlate with poor prognosis, suggesting it's a potential therapeutic target for OSCC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Oral squamous cell carcinoma (OSCC) has a poor prognosis, with 90% of head and neck cancers.
- MicroRNA-150 (miR-150) is typically downregulated in cancers and functions as a tumor suppressor.
- Understanding miR-150's role in OSCC is crucial for developing new treatments.
Purpose of the Study:
- To investigate the functional role of miR-150 in oral squamous cell carcinoma (OSCC).
- To determine the relationship between miR-150 and HMGA2 in OSCC progression.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to measure miR-150 and HMGA2 expression.
- Cell viability (MTT) and invasion (Transwell) assays to assess OSCC cell behavior.
- Western blot for epithelial-mesenchymal transition (EMT) markers and luciferase reporter assay to confirm direct binding of miR-150 to HMGA2.
Main Results:
- MiR-150 was significantly downregulated, while HMGA2 was upregulated in OSCC tissues and cells.
- Low miR-150 or high HMGA2 expression predicted poor patient survival.
- Overexpression of miR-150 inhibited OSCC cell viability, invasion, and EMT by targeting HMGA2.
- HMGA2 partially reversed the inhibitory effects of miR-150 on OSCC cell viability and invasion.
Conclusions:
- MiR-150 suppresses OSCC progression by inhibiting cell viability, invasion, and EMT through direct targeting of HMGA2.
- MiR-150 is a critical regulator in OSCC progression.
- MiR-150 shows potential as a diagnostic marker and therapeutic target for OSCC.
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