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Release of immunoreactive-neuropeptide by rat platelets
A K Myers1, M Y Farhat, C A Vaz
1Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, DC 20007.
Biochemical and Biophysical Research Communications
|August 30, 1988
Summary
Platelets release Neuropeptide Y (NPY) during aggregation, suggesting they are a significant source of this peptide in circulation. This platelet-derived NPY may influence blood pressure and heart function in certain conditions.
Area of Science:
- Cardiovascular Physiology
- Hematology
- Neuroendocrinology
Background:
- Neuropeptide Y (NPY) is a known vasoconstrictor and cardiac depressant released from sympathetic nerve endings.
- The presence of NPY in megakaryocytes suggests potential storage and release from platelets.
Purpose of the Study:
- To investigate if platelets store and release Neuropeptide Y.
- To determine the relationship between platelet aggregation and NPY release.
Main Methods:
- Measurement of immunoreactive-neuropeptide Y in platelet-rich and platelet-poor plasma.
- Induction of platelet aggregation using collagen and adenosine diphosphate (ADP).
- Quantification of NPY release in response to platelet activation.
Main Results:
- Significantly higher NPY levels were found in platelet-rich plasma compared to platelet-poor plasma.
- Collagen-induced platelet aggregation led to a concentration-dependent release of NPY.
- ADP induced minimal NPY release, correlating with aggregation phases that do not involve platelet degranulation.
Conclusions:
- Platelet release is identified as a major source of circulating Neuropeptide Y.
- Platelet activation and subsequent NPY release may play a role in the hemodynamics of pathophysiological states.