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Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

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Microfluidic processing of stem cells for autologous cell replacement.

Nicholas E Stone¹, Andrew P Voigt², Robert F Mullins²

¹The George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.

Stem Cells Translational Medicine

|June 22, 2021

Summary

Induced pluripotent stem cells (iPSCs) offer a promising source for photoreceptor cell replacement therapy for inherited retinal blindness. This review explores cell sorting and genetic correction methods, including novel microfluidic devices, for clinical applications.

Keywords:

autologous stem cell transplantation induced pluripotent stem cells retina retinal photoreceptors stem/progenitor cell

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Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

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Area of Science:

Ophthalmology
Regenerative Medicine
Stem Cell Biology

Background:

Inherited retinal degenerative diseases cause blindness.
Autologous photoreceptor cell replacement using induced pluripotent stem cells (iPSCs) is a promising therapeutic strategy.
Current differentiation protocols yield mixed retinal cell populations, necessitating photoreceptor enrichment.

Purpose of the Study:

To review cell sorting and genetic modification techniques for enhancing photoreceptor cell replacement therapy.
To evaluate existing and novel microfluidic devices for clinical-grade photoreceptor cell production.
To discuss the integration of these technologies into the clinical manufacturing pipeline.

Main Methods:

Literature review of cell sorting strategies (e.g., cell sorting, transfection).
Focus on next-generation microfluidic devices for cell enrichment and genetic correction.
Analysis of advantages and disadvantages of various approaches.

Main Results:

Induced pluripotent stem cells (iPSCs) can generate large numbers of photoreceptor cells.
Photoreceptor enrichment is crucial for successful cell replacement therapy.
Genetic correction of mutations is necessary to prevent disease recurrence.
Microfluidic devices show potential for efficient and cost-effective cell processing.

Conclusions:

Optimized cell sorting and genetic correction are essential for autologous photoreceptor cell replacement.
Novel microfluidic platforms can streamline clinical-grade cell production.
Integrating these technologies may reduce cost and regulatory hurdles for treating inherited retinal blindness.