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A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
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Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
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CD133+/CD44+ prostate cancer stem cells exhibit embryo-like behavior patterns.

Eda Acikgoz1, Burak Cem Soner2, Berrin Ozdil3

  • 1Van Yuzuncu Yil University, Faculty of Medicine, Department of Histology and Embryology, Van, 65080, Turkey.

Acta Histochemica
|June 22, 2021
PubMed
Summary

Cancer stem cells (CSCs) can form unique embryo-like spheroids. These structures suggest a link between embryonic development and prostate cancer, potentially revealing new therapeutic targets.

Keywords:
Blastula-like structureCancer stem cellsGiant cancer cellsHollow sphereProstate cancerTumor spheroids

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Area of Science:

  • Oncology
  • Developmental Biology
  • Cell Biology

Background:

  • Cancer stem cells (CSCs) drive tumor initiation, metastasis, and recurrence.
  • CSCs exhibit properties linking cancer formation to embryonic development.
  • Prostate cancer research is crucial for understanding tumor heterogeneity.

Purpose of the Study:

  • To investigate the spheroid formation of prostate cancer stem cells (CSCs).
  • To explore the embryonic-like properties of CSC-derived spheroids.
  • To identify potential therapeutic targets based on CSC behavior.

Main Methods:

  • Isolation of CD133+/CD44+ prostate CSCs from the DU-145 cell line using FACS.
  • Formation of prostate spheroids in 3D culture using agarose-coated plates.
  • Histochemical analysis of spheroid morphology, Ki-67, and Caspase-3 expression.

Main Results:

  • CD133+/CD44+ prostate CSCs formed distinct spheroid structures, including those with transparent outer zones.
  • Hollow, blastula-like spheroids were observed, mimicking early embryonic structures.
  • These findings suggest a reiteration of embryonic factor expression in CSCs.

Conclusions:

  • Prostate CSCs can form embryo-like structures, indicating a potential link to developmental processes.
  • The formation of transparent zones and hollow spheres warrants further investigation.
  • Understanding these CSC behaviors may lead to novel therapeutic strategies for prostate cancer.