Related Experiment Video
Updated: Nov 1, 2025

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
Voltametric Determination of Zoledronic Acid in a Pharmaceutical Formulation
Abdulaziz Amro1, Samer Ratrout2, Fadi Asfour2
1Al-Ahliyya Amman University Faculty of Pharmacy, Department of Pharmaceutical Sciences, Amman, Jordan
Objectives:
The aim of this study is to study the electroactivity of zoledronic acid (ZOL), optimize the parameters affecting voltametric analysis of ZOL, and make a comparison between voltametric methods used to assay ZOL.
Materials And Methods:
Three voltametric methods, cyclic voltammetry (CV), square wave voltammetry (SWV), and differential pulse voltammetry (DPV), were used to determine the concentrations of ZOL solutions (0.25-1.2 mg.mL-1). Britton-Robinson universal buffer solutions (BRB) were used as supporting electrolytes with a glassy carbon working electrode.
Results:
The calibration plots were linear in the range from 0.20 to 1.2 mg.mL-1 for differential DPV and CV and from 0.09 to 1.2 mg.mL-1 for SWV. DPV showed the highest correlation coefficient R2 value of 0.993 and the lowest limit of detection (LOD) of 37.2 μg.mL-1. Furthermore, DPV exhibited the highest precision with the lowest relative standard deviations (RSD) values. For a commercial product of ZOL, DPV showed the best accuracy and precision with 102.32% recovery and 2.88% RSD.
Conclusion:
ZOL is an electroactive compound. The pH of the BRB supporting the electrolyte is important for ZOL electroactivity. DPV is the recommended method for voltametric analysis of ZOL because of its high-performance regarding accuracy, precision, and LOD compared with other studied methods.
Related Concept Videos
Voltammetry: Overview
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
Voltammetry: Factors Affecting Measurements
Voltammetric Techniques: Linear-Scan (E vs Time)
Voltammetric Techniques: Pulse Voltammetry
Measurement of Bioavailability: Pharmacodynamic Methods
EDTA: Indirect and Alkalimetric Titration

