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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Nov 1, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

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Interpreting a lateral flow SARS-CoV-2 antigen test

Oliver T Mytton^1,2, Noel McCarthy³, Jessica Watson⁴

¹Milton Keynes Council, Milton Keynes, UK oliver.mytton@milton-keynes.gov.uk.

BMJ (Clinical Research Ed.)

|June 23, 2021

Summary

No abstract available in PubMed .

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