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Updated: Nov 1, 2025
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
Downregulation of miR-145 is associated with perineural invasion in penile carcinoma
Jaqueline Diniz Pinho1,2,3, Gyl Eanes Barros Silva2,4, Antonio Augusto Lima Teixeira Júnior2,5
1University State of Maranhão, Zé Doca, MA, Brazil.
Background:
Human papillomavirus (HPV) infection is a risk factor for penile cancer (PC). The miR-145 expression has been correlated to this virus genomic amplification. In this context, this work aims to determine the expression level of miR-145 in penile tumors infected by high-risk HPV and correlate it with the clinicopathological characteristics of the tumor and protein expression of p53.
Methods:
Formalin-fixed paraffin-embedded from 52 patients with PC, at diagnosis and prior to any cancer treatment, were obtained. HPV identification was performed by nested type PCR, and miR-145 expression was obtained by qRT-PCR. Immunohistochemical analysis of p53 and Ki-67 was performed.
Results:
Tumoral miR-145 expression was significantly lower compared to adjacent tissue. Additionally, there was a significant reduction of miR-145 expression in invasion perineural, histological associated HPV, and absence of p53 expression in positive HPV cases. HPV infection was detected in 86.5%, the most frequent HPV16. Reduced disease-free survival was observed in patients with low expression of miR-145.
Conclusions:
Our data suggest that the underexpression of miR-145 may be triggered by HPV action, decreasing protein expression of p53, and being correlated with perineural invasion. Therefore, the deregulation of miR-145 provides clues as to the potential role in penile carcinogenesis and is also a potential candidate for validation in noninvasive samples.
Insights
Low miR-145 expression in penile tumors is linked to human papillomavirus (HPV) infection and poorer survival. This suggests miR-145 deregulation plays a role in penile cancer development.
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- Human papillomavirus (HPV) infection is a known risk factor for penile cancer (PC).
- miR-145 expression has been linked to HPV genomic amplification.
- Understanding miR-145's role in HPV-associated PC is crucial.
Purpose of the Study:
- To determine miR-145 expression levels in penile tumors with high-risk HPV.
- To correlate miR-145 levels with clinicopathological features and p53 protein expression.
- To investigate miR-145's potential role in penile carcinogenesis.
Main Methods:
- Analysis of 52 penile cancer (PC) patient samples.
- HPV detection using nested type PCR.
- Quantification of miR-145 expression via qRT-PCR.
- Immunohistochemical assessment of p53 and Ki-67 protein expression.
Main Results:
- Tumoral miR-145 expression was significantly lower than in adjacent tissues.
- Reduced miR-145 expression correlated with perineural invasion and HPV presence.
- Low miR-145 expression was associated with reduced disease-free survival.
- HPV, predominantly HPV16, was detected in 86.5% of cases.
Conclusions:
- HPV infection may trigger miR-145 underexpression, impacting p53 protein levels.
- Deregulation of miR-145 is implicated in penile carcinogenesis.
- miR-145 is a potential biomarker for penile cancer, possibly detectable in noninvasive samples.
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