Microbial Azurin Immobilized on Nano-Chitosan as Anticancer and Antibacterial Agent Against Gastrointestinal Cancers
Nawal E Al-Hazmi1, Deyala M Naguib2,3
1Department of Chemistry, Division of Biology (Microbiology), University College of Qunfudhah, Umm Al-Qura University, Qunfudhah, Saudi Arabia.
Objectives:
To discover new natural effective anticancer agents and new antibacterial agents against antibiotic-resistant bacteria which are the most serious public health concern. Another important concern is drug delivery which is the transport of pharmaceutical compounds to have a therapeutic effect in organisms having a disease. Azurin is a promising anticancer agent produced from Pseudomonas aeruginosa. This study tried to test the effectiveness of the immobilization of azurin on nano-chitosan to enhance its anticancer and antibacterial activity against gastrointestinal cancer and its related bacteria.
Methods:
We purified azurin protein from Pseudomonas aeruginosa and then immobilized it on nano-chitosan. The anticancer activity of the free and nano-azurin is tested against a gastric cancer cell line (CLS-145), pancreatic cancer cell line (AsPC-1), colon cancer cell line (HCT116), esophagus cancer cell line (KYSE-410), and liver cancer cell line (HepG2). The antibacterial activity of both free and immobilized azurin also is tested against bacterial species related to the gastrointestinal cancer biopsies: Helicobacter pylori, Bacteroides fragilis, Salmonella enterica, Fusobacterium nucleatum, and Porphyromonas gingivalis.
Results:
Both free and nano-azurin showed high anticancer and antibacterial activity. Immobilization significantly increased the anticancer and antibacterial activity of the azurin CONCLUSION: Nano-azurin can be used as an effective anticancer and antibacterial agent against gastrointestinal cancer and bacterial species related to these cancers.
Insights
Researchers developed nano-azurin by immobilizing azurin protein on nano-chitosan. This enhanced nano-azurin exhibits potent anticancer and antibacterial properties, offering a promising new agent against gastrointestinal cancers and resistant bacteria.
Area of Science:
- Biotechnology
- Nanomedicine
- Antimicrobial Research
Background:
- Antibiotic-resistant bacteria and gastrointestinal cancers pose significant public health challenges.
- Azurin, a protein from Pseudomonas aeruginosa, shows potential as an anticancer agent.
- Effective drug delivery systems are crucial for therapeutic efficacy.
Purpose of the Study:
- To explore the potential of immobilizing azurin on nano-chitosan to enhance its anticancer and antibacterial activities.
- To evaluate nano-azurin's efficacy against various gastrointestinal cancer cell lines.
- To assess nano-azurin's effectiveness against key bacteria associated with gastrointestinal cancers.
Main Methods:
- Purification of azurin protein from Pseudomonas aeruginosa.
- Immobilization of azurin onto nano-chitosan.
- In vitro testing of anticancer activity against gastric, pancreatic, colon, esophageal, and liver cancer cell lines.
- In vitro testing of antibacterial activity against Helicobacter pylori, Bacteroides fragilis, Salmonella enterica, Fusobacterium nucleatum, and Porphyromonas gingivalis.
Main Results:
- Both free azurin and nano-azurin demonstrated significant anticancer and antibacterial activities.
- Immobilization of azurin on nano-chitosan notably amplified its anticancer and antibacterial efficacy.
- Nano-azurin proved effective against all tested gastrointestinal cancer cell lines and bacterial species.
Conclusions:
- Nano-azurin represents a promising therapeutic agent for combating gastrointestinal cancers.
- Nano-azurin shows potential as an effective antibacterial agent against drug-resistant bacteria linked to gastrointestinal conditions.
- Immobilization on nano-chitosan is a viable strategy to enhance the therapeutic potential of azurin.
Related Concept Videos
01:24Microbial Corrosion
01:24iChip