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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometric Analysis of Glycosphingolipid Antigens
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MS-based glycomics and glycoproteomics methods enabling isomeric characterization.

Wenjing Peng1, Cristian D Gutierrez Reyes1, Sakshi Gautam1

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas, USA.

Mass Spectrometry Reviews
|June 23, 2021
PubMed
Summary

Glycosylation, a key cell modification, impacts health and disease. This review details mass spectrometry and separation techniques for analyzing complex glycan and glycopeptide isomers, crucial for understanding disease.

Keywords:
MS-based isomeric characterizationglycan isomersglycopeptide isomers

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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Glycomics and Proteomics

Background:

  • Glycosylation is a vital posttranslational modification influencing numerous cellular functions and protein stability.
  • Aberrant glycosylation patterns are linked to diseases like cancer, diabetes, and Alzheimer's.
  • Analyzing glycan and glycopeptide isomers is challenging due to glycosylation's microheterogeneity.

Purpose of the Study:

  • To review current mass spectrometry (MS)-based techniques for glycan and glycopeptide isomer characterization.
  • To discuss separation methods coupled with MS for enhanced isomer analysis.
  • To highlight the role of bioinformatics in processing complex glycomic and glycoproteomic data.

Main Methods:

  • Utilized mass spectrometry (MS) with various fragmentation techniques for structural information.
  • Employed separation techniques such as liquid chromatography, capillary electrophoresis, and ion mobility prior to MS.
  • Integrated separation methods with MS for improved isomer identification and quantification.

Main Results:

  • Mass spectrometry alone presents challenges in comprehensive glycan and glycopeptide isomer characterization.
  • Hyphenated techniques combining separation methods with MS significantly improve isomer resolution and analysis.
  • Bioinformatic tools are crucial for automated data processing in large-scale glycomic and glycoproteomic studies.

Conclusions:

  • Effective characterization of glycan and glycopeptide isomers requires a combination of advanced separation techniques and mass spectrometry.
  • These analytical strategies are essential for advancing our understanding of glycosylation's role in health and disease.
  • Continued development of analytical and computational tools will facilitate deeper insights into glycomic and glycoproteomic complexity.