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Updated: Nov 1, 2025
A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
Yao-Yao Zhang1, Mattia Ghirardello2, Ting Wang1
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, 210095 Nanjing, China. josef.voglmeir@njau.edu.cn.
A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.
A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.
