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Related Experiment Video

Updated: Nov 1, 2025

Quantification of Circular RNAs Using Digital Droplet PCR
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Quantitation of Long Noncoding RNA Using Digital PCR.

Yu Ota1, Irene K Yan1, Tushar Patel1

  • 1Mayo Clinic, Jacksonville, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2021
PubMed
Summary
This summary is machine-generated.

Digital PCR (dPCR) offers sensitive, absolute quantification of long noncoding RNAs (lncRNAs), crucial for disease biomarker research. This method accurately measures low-abundance lncRNA expression without normalization.

Keywords:
Digital PCRGene expressionLong noncoding RNA

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Long noncoding RNAs (lncRNAs) play roles in physiological and disease processes.
  • Altered lncRNA expression is linked to disease development.
  • Accurate lncRNA quantitation is vital for biomarker discovery, especially in circulation.

Purpose of the Study:

  • To highlight the utility of digital PCR (dPCR) for sensitive lncRNA quantitation.
  • To address the challenges of measuring low-abundance lncRNA transcripts.
  • To present dPCR as a method for accurate gene expression measurement.

Main Methods:

  • Utilized digital polymerase chain reaction (dPCR) for absolute quantification of lncRNAs.
  • Focused on sensitive detection technologies for low-expression targets.
  • Explored dPCR's capability for measuring gene expression at low transcript levels.

Main Results:

  • dPCR provides highly sensitive detection of lncRNAs.
  • Absolute quantification of lncRNA is achievable with dPCR.
  • dPCR offers advantages for quantifying low-abundance targets.

Conclusions:

  • Digital PCR is a sensitive and accurate method for lncRNA quantitation.
  • dPCR is valuable for measuring low-expression lncRNAs as potential disease biomarkers.
  • The direct measurement capability of dPCR simplifies low-abundance target analysis.