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Updated: Nov 1, 2025

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Published on: May 30, 2025
In Vivo Silencing/Overexpression of lncRNAs by CRISPR/Cas System
Marianna Vitiello1,2, Laura Poliseno3,4, Pier Paolo Pandolfi5,6
1Oncogenomics Unit, CRL-ISPRO, Pisa, Italy.
Abstract:
Long noncoding RNAs (lncRNAs) are implicated in several biological processes and it has been observed that their expression is altered in several diseases. The generation of animal models where selective silencing or overexpression of lncRNAs can be attained is crucial for their biological characterization, since it offers the opportunity to analyze their function at the tissue specific or organismal level. CRISPR/Cas technology is a newly developed tool that allows to easily manipulate the mouse genome, in turn allowing to discover lncRNAs functions in an in vivo context. Here, we provide an overview of how CRISPR/Cas technology can be used to generate transgenic mouse models in which lncRNAs can be studied.
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