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lncRNA - Long Non-coding RNAs02:39

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Experimental Validation of the Noncoding Potential for lncRNAs.

Emily A Dangelmaier1, Ashish Lal2

  • 1Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2021
PubMed
Summary

Long noncoding RNAs (lncRNAs) are increasingly found to encode proteins, necessitating accurate coding potential assessment. This study details experimental techniques for determining lncRNA coding potential and function.

Keywords:
Coding potentialLong noncoding RNAsMicropeptidesTranslationlncRNAs

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Long noncoding RNAs (lncRNAs) are recognized as key regulators in cellular processes.
  • Proteomic advancements reveal that many lncRNAs are misannotated and can encode proteins.
  • Accurate determination of lncRNA coding potential is crucial before functional studies.

Purpose of the Study:

  • To detail experimental techniques for assessing the coding potential of lncRNAs.
  • To highlight methods applicable to both coding potential determination and functional analysis of lncRNAs.

Main Methods:

  • Experimental techniques for lncRNA coding potential determination.
  • Proteomic analysis.
  • RNA and protein-based functional assays.

Main Results:

  • Numerous experimental methods can accurately determine lncRNA coding potential.
  • Several techniques serve dual purposes for both coding potential and functional studies.
  • The findings emphasize the dual nature of lncRNAs as both RNA and protein-coding molecules.

Conclusions:

  • Accurate assessment of lncRNA coding potential is essential for understanding their biological roles.
  • The presented methods offer a comprehensive toolkit for researchers studying lncRNA biology.
  • Future research should consider the protein-coding capacity of lncRNAs in functional investigations.