Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bias-compensated Q-learning for optimal tracking control under denial-of-service attacks.

ISA transactions·2026

Same author

Endogenous Sulfane Sulfur Mediates the Oxidative Stress Response Process in <i>Pseudomonas aeruginosa</i>.

Antioxidants (Basel, Switzerland)·2026

Same author

A One-Step, Cell-Ready Collagen Bioink Enabling DLP Bioprinting and Enhanced Healing of Diabetic Wounds.

Biomacromolecules·2026

Same author

Laparoscopic surgery for subcapsular hematoma of the liver with preeclampsia: a case report.

Gynecology and pelvic medicine·2026

Same author

Serpine1 regulates Th17 cell differentiation and exacerbates IMQ-induced skin inflammation.

Journal of immunology (Baltimore, Md. : 1950)·2026

Same author

First detection of IncHI2 plasmid carrying bla<sub>NDM-5</sub> in Escherichia coli from retail shellfish.

Food research international (Ottawa, Ont.)·2026

Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026

Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026

Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026

Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026

Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026

Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 1, 2025

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

Published on: July 14, 2017

Advances in Automated Piston Liquid-Liquid Microextraction Technique.

Ronda Gras¹, Guangyu Liu², Robert A Shellie³

¹Dow Canada ULC, PO Bag 16, Hwy 15, Fort Saskatchewan, AB, T8L 2P4, Canada; Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Private Bag 75 Hobart, 7001 SA.

Journal of Chromatography. A

|June 23, 2021

Summary

This summary is machine-generated.

A novel automated micro liquid-liquid extraction method offers rapid, efficient, and repeatable sample preparation. This environmentally friendly technique minimizes organic solvent use and is ideal for high-throughput laboratories.

Keywords:

1,4-dioxane Emulsion-forming Liquid-liquid extraction Mixxor demulsifier gas chromatography

More Related Videos

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments

Published on: January 23, 2018

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Published on: August 3, 2016

Related Experiment Videos

Last Updated: Nov 1, 2025

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

Published on: July 14, 2017

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments

Published on: January 23, 2018

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Published on: August 3, 2016

Area of Science:

Analytical Chemistry
Separation Science

Background:

Traditional liquid-liquid extraction methods can be time-consuming and solvent-intensive.
There is a need for faster, more efficient, and environmentally friendly extraction techniques in analytical laboratories.

Purpose of the Study:

To develop and validate a novel automated micro liquid-liquid extraction technique.
To assess the efficiency, speed, repeatability, and solvent consumption of the new method.

Main Methods:

A syringe-based automated micro liquid-liquid extraction system was developed.
The technique utilizes vigorous fluid agitation and shearing effects for enhanced extraction.
Method performance was evaluated using model compounds like 1,4-dioxane, phenolics, chloroform, and hexane in aqueous matrices.

Main Results:

The new technique demonstrated extraction speeds at least 20 times faster than mechanical shaking or sonication.
Excellent repeatability was achieved, with relative standard deviations as low as 0.56% for 1,4-dioxane.
The method effectively handles emulsion-forming samples and minimizes organic solvent use to as low as 200 µL.

Conclusions:

The developed automated micro liquid-liquid extraction technique is highly efficient, rapid, and repeatable.
It offers significant advantages in terms of speed, reduced solvent consumption, and suitability for complex samples.
The technique is well-suited for automated, high-throughput, and cost-effective quality assurance laboratories.