miR-593-3p Promotes Proliferation and Invasion in Prostate Cancer Cells by Targeting ADIPOR1

Accumulating evidence has indicated that dysregulation of microRNAs (miRNAs) contributes to the tumorigenesis of prostate cancer (PCa). Nevertheless, the role of miR-593-3p in the development of PCa remains unclear. The objective of this study was to investigate the role and mechanisms of miR-593-3p in PCa cells.

RT-PCR was used to detect the expression levels of miR-593-3p. CCK-8, colony formation, spheroid formation and transwell assays were performed to examine the proliferation, migration and invasion of C4-2, DU145 and RWPE-1 cells. And then, transcriptome sequencing, dual-luciferase reporter assay and Western blot were taken to identify the target gene and downstream mechanisms of miR-593-3p.

Here, we found that miR-593-3p promoted PCa cell proliferation, colony formation, spheroid formation, migration and invasion. Further mechanistic studies revealed that miR-593-3p possessed binding sites of ADIPOR1 3'-UTR and played an important role in 5'-AMP-activated protein kinase (AMPK) signaling pathway and epithelial-mesenchymal transition (EMT) process. In addition, the transfection of si-ADIPOR1 also enhanced the PCa cell proliferation and invasion.

Our study provides an empirical investigation of miR-593-3p, which exerts oncogenic function through targeting ADIPOR1 in PCa cells.