Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.7K
Photoluminescence: Applications01:14

Photoluminescence: Applications

617
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
617
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.4K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of divalent galactosyl and fucosyl spiropyran derivatives for the targeted inhibition of bacterial biofilms.

Chemical communications (Cambridge, England)·2026
Same author

A novel water-soluble near-infrared fluorescent probe for monitoring viscosity fluctuations in plants and zebrafish under abiotic stresses.

Smart molecules : open access·2026
Same author

Spatiotemporal analysis of reactive oxygen species using specific activity-based sensing fluorescent imaging probes.

Nature reviews. Chemistry·2026
Same author

Artificial intelligence-assisted phenotyping of drug-resistant bacteria using a monosaccharide-based fluorescent sensor array.

Chemical science·2026
Same author

A Thiophilic Hg<sup>2+</sup>-Activated Fluorescent Probe for Multiscale Bioimaging: From Living Cells and Mice to Tomato Plants.

Analytical chemistry·2026
Same author

The design and development of glucose probes for sensing and imaging within biological systems.

Chemical Society reviews·2026

Related Experiment Video

Updated: Nov 1, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

665

Fluorescent small organic probes for biosensing.

Xue Tian1, Lloyd C Murfin1, Luling Wu1

  • 1Department of Chemistry, University of Bath Bath BA2 7AY UK lw960@bath.ac.uk s.e.lewis@bath.ac.uk t.d.james@bath.ac.uk.

Chemical Science
|June 24, 2021
PubMed
Summary
This summary is machine-generated.

Small-molecule fluorescent probes offer simple, sensitive detection of biological molecules. This review covers key sensing mechanisms and future directions for advanced biosensing applications.

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.4K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.8K

Related Experiment Videos

Last Updated: Nov 1, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

665
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.4K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.8K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Imaging

Background:

  • Small-molecule fluorescent probes are crucial for detecting and imaging biological signaling molecules.
  • These probes offer advantages like simplicity, high selectivity, sensitivity, and non-invasive real-time analysis of living systems.

Purpose of the Study:

  • To review and highlight key sensing mechanisms for small-molecule fluorescent probes.
  • To discuss challenges and suggest future development directions for improved biosensing probes.

Main Methods:

  • Review of established fluorescence sensing mechanisms.
  • Discussion of Förster resonance energy transfer (FRET), intramolecular charge transfer (ICT), photoinduced electron transfer (PeT), excited state intramolecular proton transfer (ESIPT), and aggregation-induced emission (AIE).
  • Exploration of dual/triple sensing mechanisms (DSM/TSM) and multi-modality approaches.

Main Results:

  • Detailed overview of various fluorescence sensing mechanisms.
  • Identification of current challenges in small-molecule fluorescent probe development.
  • Proposed future research directions for enhanced probe performance.

Conclusions:

  • Small-molecule fluorescent probes are vital tools in biosensing.
  • Further development is needed to overcome existing challenges and improve probe capabilities for advanced biological applications.