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HTR7 promotes laryngeal cancer growth through PI3K/AKT pathway activation

Xiaoli Sheng1,2, Wenlin Liu2,3, Zhongming Lu1,2

  • 1Department of Otorhinolaryngology, Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences, Guangzhou, China.

Annals of Translational Medicine
|June 24, 2021
PubMed
Abstract

Insights

The 5-hydroxytryptamine receptor 7 (HTR7) is upregulated in laryngeal cancer, promoting tumor growth by activating the PI3K/AKT pathway. HTR7 serves as both a therapeutic target and a prognostic factor for this cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • G protein-coupled receptors (GPCRs) research

Background:

  • Laryngeal cancer is a significant head and neck malignancy requiring novel therapeutic targets.
  • The 5-hydroxytryptamine receptor 7 (HTR7), a druggable GPCR, has an unknown role in laryngeal cancer.

Purpose of the Study:

  • To investigate the role and mechanism of HTR7 in laryngeal cancer.
  • To evaluate HTR7 as a potential therapeutic target and prognostic biomarker.

Main Methods:

  • Cell proliferation assays (colony formation, soft agar, BrdU, MTT) and xenograft models assessed HTR7's effect on tumor growth.
  • Luciferase reporter assays examined HTR7's impact on the PI3K/AKT pathway.
  • Cox regression models analyzed HTR7 expression as a prognostic factor.

Main Results:

  • HTR7 expression is significantly upregulated in laryngeal cancer tissues and cells.
  • High HTR7 expression correlates with shorter patient survival and is an independent prognostic factor.
  • HTR7 overexpression promotes laryngeal cancer proliferation and growth, while knockdown inhibits it.
  • HTR7 activates the PI3K/AKT pathway, driving tumor growth.

Conclusions:

  • HTR7 plays a crucial role in promoting laryngeal cancer progression.
  • HTR7 is a promising therapeutic target for laryngeal cancer.
  • HTR7 serves as a valuable prognostic biomarker for laryngeal cancer patients.
Keywords:
5-hydroxytryptamine receptor 7laryngeal cancerprotein kinase B (AKT)tumor growth

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