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Updated: Nov 1, 2025

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

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Long non-coding RNA X-inactive specific transcript promotes osteosarcoma metastasis via modulating microRNA-758/Rab16

Wei Liu1, Qiuping Long1, Li Zhang1

  • 1Department of Orthopedics Trauma, Nanhua Hospital Affiliated to Nanhua University, Hengyang, China.

Annals of Translational Medicine
|June 24, 2021
PubMed
Abstract

Insights

Long non-coding RNA XIST promotes osteosarcoma metastasis by regulating microRNA-758 and Rab16. This study reveals a novel mechanism driving cancer spread in bone sarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is a prevalent bone sarcoma impacting numerous lives.
  • Understanding the molecular mechanisms of OS metastasis is crucial for effective treatment.

Purpose of the Study:

  • To investigate the roles of long non-coding RNA XIST and microRNA-758 in osteosarcoma metastasis.
  • To identify the downstream effector of the XIST/miR-758 axis in OS.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to assess XIST and miR-758 expression.
  • Cell transfection to manipulate XIST and miR-758 levels, followed by assays for cell viability, migration, and invasion.
  • Dual-luciferase reporter assay to confirm regulatory interactions between XIST, miR-758, and Rab16.

Main Results:

  • XIST was upregulated, while miR-758 was downregulated in OS tissues and cells.
  • XIST overexpression and miR-758 inhibition promoted OS cell migration, invasion, and epithelial-mesenchymal transition (EMT).
  • XIST directly regulated miR-758, which in turn regulated Rab16; Rab16 overexpression counteracted miR-758 mimic effects.

Conclusions:

  • The lncRNA XIST facilitates osteosarcoma cell migration, invasion, and EMT.
  • XIST exerts its pro-metastatic effects through the miR-758/Rab16 signaling pathway.
Keywords:
Osteosarcoma (OS)Rab16lncRNA X-inactive specific transcript (lncRNA XIST)microRNA-758tumor metastasis

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