Related Experiment Video

Updated: Nov 1, 2025

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

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Where in the cell is my protein?

David J DeRosier1

  • 1Brandeis University, Waltham, MA, USA.

Quarterly Reviews of Biophysics
|June 24, 2021
PubMed
Summary

Cryo-correlative light and cryo-electron microscopy (cryo-CLEM) uses fluorescent tags for precise structure localization. Enhancements like cryo-single-molecule localization microscopy (cryo-SMLM) improve accuracy but face challenges in imaging and instrumentation.

Keywords:
Cryo-CLEMFIB-SEM microscopycryo-ETcryo-electron microscopycryo-superresolution fluorescence microscopylocalization of structures

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