A Strong Dual-Component Bioadhesive Based on Solventless Thiol-isocyanate Click Chemistry

Isocyanate is an efficient tissue anchor for engineering of strong bioadhesives. However, isocyanate-containing adhesives were seldom manufactured due to their requirement of water-free administration and time-consuming moisture-induced solidification. To address this issue, here, a solventless dual-component bioadhesive based on thiol-isocyanate cross-linking chemistry is reported. This dual-component bioadhesive consists of a hyperbranched polymer with thiol groups (HBPTE) and an isocyanate-modified polyethylene glycol (PEGNCO). HBPTE and PEGNCO are low-viscosity fluids at room temperature and hence could be used directly as adhesive components, in the absence of a catalyst and a solvent. The thiol-isocyanate click chemistry of components provides the HBPTE-PEGNCO mixture with a gelation time of 1.8-3 min, which makes it acceptable for practical applications. The abundance of isocyanate groups in the adhesive molecule provides strong bonding strength through formation of chemical linkages with reactive groups on the tissue. Moreover, in vitro and in vivo evaluations showed excellent biocompatibility of the HBPTE-PEGNCO adhesive. This dual-component bioadhesive based on solventless thiol-isocyanate click chemistry displayed a fast gelation time and excellent bonding performance, providing a pioneering idea for engineering isocyanate-containing bioadhesives.