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Updated: Nov 1, 2025

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Published on: January 17, 2025

Elastolytic activity is associated with inflammation in bladder cancer.

Natalia Gruba¹, Lech Stachurski², Adam Lesner¹

¹Department of Environmental Technology, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63 Street, PL 80-308 Gdańsk, Poland.

Journal of Biochemistry

|June 24, 2021

Summary

Urine enzyme activity, specifically proteolytic and human neutrophil elastase, may indicate inflammation linked to epithelial neoplasms. This study suggests urine analysis is a viable tool for inflammation and cancer screening.

Keywords:

chromogenic substrates inflammation neoplasm

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Area of Science:

Biochemistry
Oncology
Urology

Background:

Cancer development is frequently linked to inflammation.
Late diagnosis of inflammation-associated cancers complicates treatment.
Identifying reliable biomarkers for early detection is crucial.

Purpose of the Study:

To qualitatively determine specific enzyme activity in urine.
To investigate the relationship between proteolytic activity, inflammation, and epithelial neoplasms.
To assess human neutrophil elastase activity as an indicator of inflammation in cancer patients.

Main Methods:

Utilized a panel of chromogenic substrates for enzyme activity determination.
Analyzed urine samples from patients with confirmed inflammation and/or epithelial neoplasms.
Included urine from individuals without inflammation as a control group.
Specifically measured proteolytic and human neutrophil elastase activity.

Main Results:

Detected differences in enzyme activity in urine samples from patients with inflammation and/or neoplasms compared to controls.
Demonstrated the presence of proteolytic activity in urine associated with epithelial neoplasms.
Identified human neutrophil elastase activity linked to inflammation in urine samples.
This is the first study to report elastolytic activity in bladder cancer urine.

Conclusions:

Urinary proteolytic activity may reflect neutrophil response to inflammation, potentially related to cancer.
Urine analysis shows promise for screening inflammation associated with epithelial neoplasms.
Further research could support broader clinical application of urine-based inflammation screening.