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Development of highly sensitive and rapid antigen detection assay for diagnosis of COVID-19 utilizing optical

Rikako Funabashi1, Kei Miyakawa1, Yutaro Yamaoka1,2

  • 1Department of Microbiology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan.

Journal of Molecular Cell Biology
|June 24, 2021
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.