Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Publication Guidelines for Optimized Multiparameter Immunolabeling Panels (OMIPs).

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

International microscopy facility benchmarking survey.

Journal of microscopy·2026
Same author

An Open-Source Workflow for Semi-Automated Spatial Profiling of Multiplex Immunofluorescent Images.

Current protocols·2026
Same author

Development of a 3D <i>In Vitro</i> Wound Healing Model to Assess the Effect of ADSC-EVs on Vascularization.

Tissue engineering. Part A·2025
Same author

A novel approach to digital characterisation of Tertiary Lymphoid Structures in colorectal cancer.

Frontiers in immunology·2025
Same author

Best practices for user consultation in flow cytometry shared resource laboratories.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2024
Same journal

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Current protocols·2026
Same journal

TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements.

Current protocols·2026
Same journal

High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA.

Current protocols·2026
Same journal

Recombinant Protein Expression in Rhodococcus species.

Current protocols·2026
Same journal

Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides.

Current protocols·2026
Same journal

CODEC Library Preparation From Genomic DNA.

Current protocols·2026
See all related articles

Related Experiment Video

Updated: Nov 1, 2025

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research
05:22

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

Published on: June 21, 2024

582

High-Dimensional Image Analysis using Histocytometry.

Luis Munoz-Erazo1, Alfonso J Schmidt1, Kylie M Price1

  • 1Malaghan Institute of Medical Research, Wellington, New Zealand.

Current Protocols
|June 24, 2021
PubMed
Summary
This summary is machine-generated.

Histocytometry uses computational methods for analyzing microscopy images to identify cell types and their spatial relationships. This automated technique enhances reproducibility and reduces bias in cell analysis for research.

Keywords:
cell segmentationcytometryhistocytometryimage analysisimmunophenotypingmicroscopy

More Related Videos

Automated Quantification of Hematopoietic Cell &#8211; Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

11.8K
ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
05:12

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

11.6K

Related Experiment Videos

Last Updated: Nov 1, 2025

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research
05:22

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

Published on: June 21, 2024

582
Automated Quantification of Hematopoietic Cell &#8211; Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

11.8K
ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
05:12

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

11.6K

Area of Science:

  • Computational Biology
  • Microscopy Image Analysis
  • Cellular Phenotyping

Background:

  • Histocytometry automates multiparameter microscopy image analysis.
  • It enables spatial analysis of cellular phenotypes and their relationships.
  • Increased availability of software democratizes complex cell analysis.

Purpose of the Study:

  • To provide an overview of a histocytometry pipeline.
  • To guide researchers in strategic planning and software selection.
  • To enable automated cell segmentation, phenotyping, and spatial analysis.

Main Methods:

  • Utilizes computational approaches for image processing.
  • Focuses on cell segmentation and phenotyping algorithms.
  • Employs spatial analysis techniques for cellular relationships.

Main Results:

  • Histocytometry offers high throughput and reproducibility.
  • Reduces user bias compared to manual methods.
  • Enables detailed spatial mapping of cell populations.

Conclusions:

  • Histocytometry pipelines empower researchers with advanced cell analysis tools.
  • Strategic planning and software are key for successful implementation.
  • This technique significantly advances research outputs through automated image analysis.