Continuous dark and photo biohydrogen production in a baffled bioreactor and electrons distribution analysis

This work studied the sequential hydrogen production by dark and photo-fermentation (HPDPF) in continuous baffled bioreactors. Taken enzymatic hydrolysate of corn stover as initial carbon source, the influence of hydraulic retention time (HRT) of dark fermentation (DF) and the dilution ratio (DR) of dark fermentation effluents (DFEs) on the hydrogen production performance of the combined fermentation system and electron distribution were investigated. For DF unit, the highest hydrogen production rate (HPR) of 5.24 L/(L·d) was detected at HRT of 18 h, however, the maximum HPR of 4.60 L/(L·d) was obtained from DFEs with HRT of 12 h and DR of 1:0.5 during photo fermentation unit, meanwhile, the electrons in substrate partitioning to H₂ reached the maximum value of 35.69%. In terms of hydrogen yield, the optimum operating conditions of the combined system were HRT of 12 h (DF) and DR of 1:0.5(DFEs), in which the hydrogen yield reached 12.73 L/d.