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Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

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Oral Biofilm Formation on Different Materials for Dental Implants

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Bifunctional Composites for Biofilms Modulation on Cervical Restorations.

A A Balhaddad^1,2, I M Garcia^1,3, L Mokeem¹

¹Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, MD, USA.

Journal of Dental Research

|June 25, 2021

Summary

This summary is machine-generated.

New dental composites with dimethylaminohexadecyl methacrylate (DMAHDM) and nanosized amorphous calcium phosphate (NACP) significantly inhibit biofilm growth. These bioactive materials show promise for improving cervical restorations and preventing plaque accumulation.

Keywords:

bioactive materials composite materials dental caries methacrylates microbiome secondary caries

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Area of Science:

Biomaterials Science
Dental Materials
Microbiology

Background:

Subgingival margins of cervical composites promote plaque accumulation and gingival irritation.
Existing dental materials lack agents to effectively combat bacterial biofilm development in acidic conditions.

Purpose of the Study:

To investigate the efficacy of novel bioresponsive bifunctional dental composites against mature subgingival biofilms.
To evaluate the potential of composites containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanosized amorphous calcium phosphate (NACP) for cervical restorations.

Main Methods:

Formulation of dental composites with varying concentrations of DMAHDM and NACP.
Analysis of composite properties including wettability and surface-free energy.
In vitro assessment of biofilm inhibition, microbial composition (16S rRNA gene sequencing), metabolic activity, and polysaccharide production.

Main Results:

DMAHDM-NACP composites exhibited higher contact angles and lower surface-free energy than controls.
Significant inhibition (3-5 log reduction) of key oral pathogens including Porphyromonas gingivalis and Fusobacterium nucleatum.
Over 80% reduction in biofilm metabolic activity and polysaccharide production was observed.

Conclusions:

The developed DMAHDM-NACP composites demonstrate potent anti-biofilm properties.
These bioactive materials show significant potential for preventing biofilm formation in cervical restorations.
The findings suggest a promising approach to enhance the longevity and performance of dental composite restorations.