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Updated: Nov 1, 2025
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
Published on: May 5, 2016
Protocol for the generation of murine bronchiolospheres
Ana Ivonne Vazquez-Armendariz1, Werner Seeger1, Susanne Herold1
1Department of Internal Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Cardio-Pulmonary Institute (CPI), Institute for Lung Health (ILH), member of the German Center for Lung Research (DZL), Justus-Liebig University Giessen, 35392 Giessen, Hessen, Germany.
Abstract:
Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth. For complete details on the use and execution of this protocol, please refer to Moiseenko et al. (2020).
