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CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2
Yiren Cao1, Jinjun Wu1, Bo Pang1
1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. hongquan@ualberta.ca xc.le@ualberta.ca.
Abstract:
The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a resulted in visual detection of the N gene and E gene of SARS-CoV-2 in 45 min.
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