Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interpretable Skin Cancer Identification Using a Hybrid Deep Learning and XAI Framework on HAM10000.

Bioengineering (Basel, Switzerland)·2026
Same author

Machine-embroidered textile electrodes: parametric engineering for lab-on-glove electrochemical pesticide detection.

Lab on a chip·2026
Same author

Nesfatin-1 mitigates calcium oxalate nephropathy in mice through GPR12 receptor modulation and PKCα/NADPH oxidase pathway inhibition.

Life sciences·2026
Same author

Capillary microfluidics aided single-cell manipulation for optical detection of bacterial pathogens.

Biophysical reviews·2026
Same author

AI-driven multimodal retinal imaging for early detection and risk stratification of vascular and neurodegenerative diseases.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
Same author

Portable Electrochemical Platform for Point-of-Care Bilirubin Sensing for Liver Diagnostics.

IEEE transactions on bio-medical engineering·2026

Related Experiment Video

Updated: Nov 1, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.2K

Miniaturized and IoT Enabled Continuous-Flow-Based Microfluidic PCR Device for DNA Amplification.

Madhusudan B Kulkarni, Srashti Goyal, Arti Dhar

    IEEE Transactions on Nanobioscience
    |June 25, 2021
    PubMed
    Summary

    A new portable, IoT-enabled microfluidic device enables rapid polymerase chain reaction (PCR) testing. This continuous-flow system achieves accurate temperatures for DNA amplification in just 32 minutes, outperforming conventional methods.

    More Related Videos

    Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
    09:36

    Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

    Published on: February 3, 2021

    5.1K
    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
    11:40

    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

    Published on: November 14, 2018

    8.8K

    Related Experiment Videos

    Last Updated: Nov 1, 2025

    Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
    14:12

    Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

    Published on: November 21, 2023

    2.2K
    Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
    09:36

    Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

    Published on: February 3, 2021

    5.1K
    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
    11:40

    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

    Published on: November 14, 2018

    8.8K

    Area of Science:

    • Biotechnology
    • Microfluidics
    • Molecular Biology

    Background:

    • Conventional polymerase chain reaction (PCR) instruments are often bulky and require significant time for DNA amplification.
    • Microfluidic devices offer potential for miniaturization and faster reaction times.
    • Developing portable, automated, and cost-effective PCR systems is crucial for point-of-care diagnostics and field applications.

    Purpose of the Study:

    • To design and fabricate a continuous-flow microfluidic device for rapid polymerase chain reaction (PCR).
    • To develop a portable, automated, and IoT-enabled thermal management system for the microfluidic PCR device.
    • To demonstrate the device's capability for efficient DNA amplification and compare its performance with conventional PCR.

    Main Methods:

    • Fabrication of a polymethyl methacrylate (PMMA) microfluidic chip with serpentine channels using CO2 laser ablation.
    • Integration of an Arduino board, cartridge heaters, and thermocouple sensors for precise temperature control (±0.2 °C) at denaturation (95 °C) and annealing (60 °C) zones.
    • Implementation of an IoT module for real-time temperature monitoring via smartphone analytics.
    • Performance evaluation using amplification of a rat GAPDH gene fragment (594 bp) and subsequent agarose gel electrophoresis.

    Main Results:

    • Successful amplification of the target DNA fragment using the microfluidic PCR device.
    • Achieved a total reaction time of 32 minutes at an optimal flow rate of 10 µL/min.
    • Demonstrated excellent performance and accuracy comparable to conventional PCR instruments.
    • The portable system is low-cost, battery-powered, and automated, with IoT capabilities for remote monitoring.

    Conclusions:

    • The developed continuous-flow microfluidic PCR device offers a rapid, portable, and accurate solution for DNA amplification.
    • The integrated IoT-enabled thermal management system enhances usability and accessibility.
    • This miniaturized system shows significant potential for advancing molecular diagnostics and research applications.