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Identification of a novel Sox5 transcript in mouse testis
Bangjin Zheng1, Chaoyang Huang2, Jian Zhou3
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
Gene Expression Patterns : GEP
|June 25, 2021
Summary
Researchers identified a novel S-Sox5 variant transcript in mouse testis, crucial for spermatogenesis. This SOX5 (SRY-related HMG box 5) isoform expression increases significantly during key developmental stages.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- SOX5 (SRY-related HMG box 5) is a transcription factor with known long (L-SOX5) and short (S-SOX5) isoforms.
- SOX5 plays a role in various biological processes, including development and cell differentiation.
Purpose of the Study:
- To identify and characterize novel transcripts of Sox5 in mouse testis.
- To investigate the expression patterns and functional domains of SOX5 isoforms during spermatogenesis.
Main Methods:
- eCLIP-based amplification of cDNA ends to identify mRNA variants.
- Semi-quantitative PCR to analyze transcript expression in mouse testis.
- In vitro transfection assays with truncated SOX5 constructs in HeLa cells.
Main Results:
- A novel S-Sox5 variant transcript with a unique 5'UTR and an additional exon 9 was identified in mouse testis.
- Both S-Sox5 variant and S-Sox5 transcripts are specifically expressed in mouse testis and increase significantly at postnatal day 21.
- Nuclear localization of SOX5 requires the N-terminus and the DNA-binding HMG domain.
Conclusions:
- The novel S-Sox5 variant is specifically expressed in mouse testis and its expression correlates with the appearance of round spermatids.
- SOX5 nuclear localization is dependent on specific protein domains, providing insights into its regulatory mechanisms.
- These findings lay the groundwork for future research into the function of SOX5 in spermatogenesis.
