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Multiplexed analytical approaches to beta-lactam allergy in vitro testing standardization
María José Juárez1, Ethel Ibáñez-Echevarria2, Dolores Hernández-Fernández de Rojas2
1Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera S/n, 46022, Valencia, Spain.
Analytica Chimica Acta
|June 26, 2021
Summary
Accurate in vitro testing for beta-lactam allergy is crucial for antibiotic stewardship and combating antimicrobial resistance. This study presents an improved multiplex immunoassay for reliable quantification of specific IgE, enhancing diagnostic accuracy.
Area of Science:
- Clinical immunology
- Microbiology
- Analytical chemistry
Background:
- Beta-lactam allergy suspicion leads to guideline-divergent antibiotic use, fueling antimicrobial resistance.
- In vitro quantification of specific IgE aids in diagnosing beta-lactam allergy and guiding antibiotic stewardship.
- Current immunoassays for specific IgE to beta-lactams face challenges due to selective immunoreagents and low serum concentrations.
Purpose of the Study:
- To develop and validate an improved, standardized multiplex microimmunoassay for in vitro detection of beta-lactam allergy.
- To enable reliable quantification of specific IgE to beta-lactam antibiotics at low concentrations.
Main Methods:
- Development of an improved multiplex microimmunoassay.
- Utilized homologous calibration for specific IgE quantification.
- Incorporated an internal standard to enhance reproducibility.
- Validated the method using 40 human serum samples.
Main Results:
- Homologous calibration enabled reliable quantification of specific IgE down to 144 ng/L.
- Use of an internal standard doubled reproducibility and achieved 93-106% recovery for penicillin and amoxicillin.
- The improved method showed excellent agreement (0.99 correlation) with a current in vitro test.
Conclusions:
- The improved multiplex microimmunoassay offers a standardized and reliable approach for beta-lactam allergy diagnosis.
- This method facilitates accurate quantification of specific IgE, supporting better antibiotic stewardship.
- Enhanced diagnostic capabilities are essential for addressing the global threat of antimicrobial resistance.
