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Updated: Nov 1, 2025
Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
Published on: October 20, 2023
Mesna ameliorates acute lung injury induced by intestinal ischemia-reperfusion in rats
Samia Adel Abd El-Baset1, Manal R Abd El-Haleem2,3, Rehab S Abdul-Maksoud4
1Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, 44519, Asharquia, Egypt. drsamia2013@yahoo.com.
Abstract:
The lung is severely affected by intestinal ischemia-reperfusion (I-R) injury. Mesna, a thiol compound, possess anti-inflammatory and antioxidant properties. We aimed in the present work to explore the potential beneficial effects of Mesna on the acute lung damage mediated by intestinal I-R in a rat model. Forty male adult albino rats were randomly separated into; control, intestinal I-R, Mesna I and Mesna II groups. Mesna was administered by intraperitoneal injection at a dose of 100 mg/kg, 60 min before ischemia (Mesna I) and after reperfusion (Mesna II). Arterial blood gases and total proteins in bronchoalveolar lavage (BAL) were measured. Lung tissue homogenates were utilized for biochemical assays of proinflammatory cytokines and oxidative stress markers. Lung specimens were managed for examination by light and electron microscopy. Our results revealed that Mesna attenuated the histopathological changes and apoptosis of the lung following intestinal I-R. Mesna also recovered systemic oxygenation. Mesna suppressed neutrophil infiltration (as endorsed by the reduction in MPO level), reduced ICAM-1 mRNA expression, inhibited NF-κB pathway and reduced the proinflammatory cytokines (TNF-α, IL-1β and IL-6) in the lung tissues. Mesna maintained the antioxidant profile as evidenced by the elevation of the tissue GPx and SOD and down-regulation of HSP70 immune-expressions. Accordingly, Mesna treatment can be a promising way to counteract remote injury of the lung resulted from intestinal I-R.
Insights
Mesna administration mitigates lung damage from intestinal ischemia-reperfusion (I-R) injury. This thiol compound reduces inflammation, oxidative stress, and apoptosis, offering a promising treatment for remote lung injury.
Area of Science:
- Physiology
- Pathology
- Pharmacology
Background:
- Intestinal ischemia-reperfusion (I-R) injury significantly impacts lung function.
- Mesna, a thiol compound, exhibits anti-inflammatory and antioxidant properties.
- Understanding Mesna's protective effects against lung damage from intestinal I-R is crucial.
Purpose of the Study:
- To investigate the potential protective effects of Mesna against acute lung damage induced by intestinal I-R in a rat model.
- To evaluate Mesna's impact on lung histopathology, oxidative stress, and inflammatory markers.
- To assess Mesna's efficacy when administered before ischemia or after reperfusion.
Main Methods:
- Intestinal I-R was induced in adult male albino rats, with Mesna administered intraperitoneally at 100 mg/kg before ischemia or after reperfusion.
- Parameters assessed included arterial blood gases, bronchoalveolar lavage (BAL) fluid protein content, lung tissue biochemical markers (proinflammatory cytokines, oxidative stress markers), and histopathological examination (light and electron microscopy).
- Neutrophil infiltration (myeloperoxidase levels), ICAM-1 mRNA expression, NF-κB pathway activation, antioxidant enzymes (GPx, SOD), and HSP70 expression were analyzed.
Main Results:
- Mesna treatment significantly attenuated lung histopathological damage and apoptosis following intestinal I-R.
- Mesna administration improved systemic oxygenation and reduced neutrophil infiltration and myeloperoxidase (MPO) levels.
- Mesna suppressed key inflammatory mediators, including ICAM-1 mRNA, NF-κB pathway activation, TNF-α, IL-1β, and IL-6, while preserving antioxidant capacity by increasing GPx and SOD and decreasing HSP70.
Conclusions:
- Mesna demonstrates significant protective effects against acute lung injury secondary to intestinal I-R.
- Mesna's therapeutic benefits stem from its anti-inflammatory and antioxidant actions, mitigating cellular damage and improving lung function.
- Mesna represents a promising therapeutic strategy for preventing or treating remote lung injury associated with intestinal I-R.
