Effect of nano-TiO₂ on humic acid utilization from piggery biogas slurry by microalgae

Resource recovery from piggery biogas slurry has become an inevitable demand for sustainable development of pig industry. Microalgae show great potential in recovering nitrogen and phosphorus from piggery slurry, but struggle to utilize organic pollutants, as most of them are inert components (e.g., humic acids, HAs). In this study, nano-TiO₂ was used to enhance the utilization of HAs by microalgae from piggery biogas slurry. Results showed that the optimal conditions for microalgal growth and HAs removal by the microalgae-TiO₂ coupling system were TiO₂ dosage of 0.30 g/L, microalgal inoculation concentration of 0.40 g/L, light intensity of 360 µmol photon/(m²·s) and temperature of 30 °C, with the microalgal chlorophyll concentration of 6.51 mg/L and HAs removal efficiency of 50.14%. Analysis of HAs composition variations in the piggery biogas slurry indicated that the decrease of HAs was caused by their decomposition into small molecules under the photocatalytic reactions of TiO₂.