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Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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Down-regulation of a panel of immune-related lncRNAs in breast cancer

Soudeh Ghafouri-Fard1, Mohammad Asadi2, Behnoush Sohrabi3

  • 1Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Pathology, Research and Practice
|June 27, 2021
PubMed

Insights

This study investigated nine immune-related long non-coding RNAs (lncRNAs) in breast cancer. Six lncRNAs were found to be downregulated in tumor tissues, suggesting their potential as novel biomarkers for breast cancer detection.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a prevalent malignancy in women, with long non-coding RNAs (lncRNAs) playing an increasingly recognized role in its pathogenesis.
  • Immune-related lncRNAs are crucial in cancer development, but some remain under-investigated in breast cancer.
  • Understanding the expression patterns of specific lncRNAs can offer insights into tumor immunity and progression.

Purpose of the Study:

  • To compare the expression levels of nine selected immune-related lncRNAs between breast cancer tissues and paired non-cancerous tissues.
  • To evaluate the potential of these lncRNAs as diagnostic biomarkers for breast cancer.
  • To explore the association between the expression of these lncRNAs and clinical characteristics of breast cancer patients.

Main Methods:

  • Gene expression analysis was performed on paired breast cancer and adjacent non-cancerous tissue samples.
  • Quantitative real-time PCR or similar expression assay was used to measure the levels of nine specific immune-related lncRNAs.
  • Statistical analyses, including differential expression analysis and receiver operating characteristic (ROC) curve analysis, were conducted to assess significance and diagnostic performance.

Main Results:

  • Six of the nine investigated lncRNAs (lnc-MICAL3-2, lnc-DDX31, LINC01063, LINC02381, ENST0000615051, and lnc-TRIM43B-1) showed significantly decreased expression in breast cancer samples compared to control tissues.
  • The expression levels of these downregulated lncRNAs were found to correlate with various clinical features of breast cancer.
  • Lnc-TRIM43B-1 demonstrated high accuracy in differentiating between tumor and non-tumor tissues, with an AUC of 0.82, sensitivity of 76%, and specificity of 73.24%.

Conclusions:

  • The identified downregulated immune-related lncRNAs may serve as potential tissue biomarkers for breast cancer diagnosis.
  • These lncRNAs' ability to distinguish between tumoral and non-tumoral tissues highlights their clinical relevance.
  • Further research into the functional roles of these lncRNAs in breast cancer immunity and progression is warranted.
Keywords:
AC009237.14AC016027.1AC083809.1AL445645.1Breast cancerENST0000615051LINC01063LINC02381Lnc-DDX31Lnc-MICAL3–2Lnc-TRIM43B-1LncRNALong non-coding RNA

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