Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Ca²⁺ imaging is the most frequently used technique to study glial cell physiology. While chemical Ca²⁺ indicators served to visualize and measure changes in glial cell cytosolic Ca²⁺ concentration for several decades, genetically encoded Ca²⁺ indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca²⁺ indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.