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Updated: Oct 31, 2025
Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
Published on: February 9, 2011
Comparison of the Prevalence of Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) among
Hossein Sedaghat1, Tahmineh Narimani2, Bahram Nasr Esfahani2
1Department of Microbiology, Faculty of Medicine, Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Background:
Staphylococcus aureus (S. aureus) is one of the most important pathogens in burn infections colonized in the nose and increase the risk of infections.
Methods:
Overall, 85 S. aureus isolates were isolated from clinical and nasal hospitalized patients and health care workers (HCWs) in a burn unit and non-burn units in Isfahan from June 2016 and September 2016. Genes encoding penicillin-binding protein 2a (mecA) and adhesive surface proteins, including fibronectin-binding proteins (fnbA,fnbB), fibrinogen binding protein (fib), laminin-binding protein(eno), collagen binding protein (cna), elastin binding protein (ebps), intracellular adhesion operon (icaA and icaD) were detected using PCR method.
Results:
The rate of methicillin-resistant S. aureus (MRSA) among burn and non-burn isolates were 62% (18/29) and 25% (14/56), respectively. The most prevalent MSCRAMMs genes in burn units were eno (86%) and fib (66%). The most common gene pattern in burn center was icaA+fib+eno. The frequency of icaD, fib and ebpS was higher in clinical samples than nasal samples. No relation was found between the MSCRAMMs genes in the burn unit and non-burn units.
Conclusion:
The high prevalence of MRSA in burn center can be a new challenge for clinicians. The higher frequency of icaD, fib and ebpS in clinical isolates than nasal isolates may reflect the important role of these genes in colonization and pathogenesis of S. aureus.
Insights
High rates of methicillin-resistant Staphylococcus aureus (MRSA) were found in burn units, posing a challenge for clinicians. Specific genes (icaD, fib, ebps) were more frequent in clinical samples, suggesting a role in S. aureus infections.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Burn Care
Background:
- Staphylococcus aureus (S. aureus) is a significant pathogen in burn wound infections.
- Nasal colonization by S. aureus increases infection risk.
- Understanding virulence factors is crucial for managing S. aureus infections.
Purpose of the Study:
- To investigate the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) in burn and non-burn units.
- To identify the presence of specific virulence genes (MSCRAMMs) in S. aureus isolates.
- To correlate gene patterns with clinical and nasal isolates.
Main Methods:
- Collected 85 S. aureus isolates from clinical and nasal samples in burn and non-burn units.
- Utilized PCR to detect genes encoding for methicillin resistance (mecA) and surface proteins (fnbA, fnbB, fib, eno, cna, ebps, icaA, icaD).
- Compared gene frequencies between burn and non-burn isolates and between clinical and nasal samples.
Main Results:
- MRSA prevalence was significantly higher in burn units (62%) compared to non-burn units (25%).
- The most common MSCRAMMs genes in burn units were eno (86%) and fib (66%), with a prevalent pattern of icaA+fib+eno.
- Genes icaD, fib, and ebps were more frequent in clinical isolates than nasal isolates.
Conclusions:
- The high MRSA rate in burn centers presents a clinical challenge.
- The increased frequency of icaD, fib, and ebps in clinical isolates suggests their importance in S. aureus colonization and pathogenesis.
- Further research is needed to understand the role of these genes in burn-related infections.
