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Related Experiment Video

Updated: Oct 31, 2025

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Published on: October 9, 2018

Micro-computed Tomography to Visualize Vascular Networks in Maize Stems.

Akiteru Maeno^1,2, Katsutoshi Tsuda^3,4

¹Mammalian Genetics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.

|June 28, 2021

Summary

We developed a micro-computed tomography (micro-CT) protocol to visualize maize stem vascular networks. This method reveals internal plant structures for better understanding of nutrient transport and plant development.

Keywords:

3D modeling Maize Micro-CT scanning Stem Vascular networks

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Published on: September 22, 2023

Area of Science:

Plant Biology
Biotechnology
Imaging Science

Background:

Plant vascular systems are crucial for transport and development.
Stem vascular networks are difficult to study due to their embedded nature in crop species.

Purpose of the Study:

To describe a protocol for visualizing maize stem vascular networks using micro-computed tomography (micro-CT).
To enable detailed study of internal plant structures and transport pathways.

Main Methods:

Sample fixation and staining with contrasting reagents.
Data acquisition using micro-CT scanning.
Reconstruction of three-dimensional (3D) models and extraction of vascular networks.

Main Results:

Successfully visualized intricate vascular networks within the maize stem.
Developed a comprehensive protocol applicable to various plant species and organs.

Conclusions:

Micro-CT scanning provides a powerful tool for non-destructively imaging plant vascular systems.
This protocol facilitates research on plant growth, development, and nutrient transport.