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Updated: Oct 31, 2025
Isolation, Culture, and Transplantation of Muscle Satellite Cells
Published on: April 8, 2014
Mouse Satellite Cell Isolation and Transplantation
Elia Angelino1,2,3, Simone Reano1,2, Michele Ferrara1,2,3
1Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy.
Abstract:
Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).
