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Updated: Oct 31, 2025

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Published on: April 8, 2014

Mouse Satellite Cell Isolation and Transplantation.

Elia Angelino^1,2,3, Simone Reano^1,2, Michele Ferrara^1,2,3

¹Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy.

|June 28, 2021

Summary

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Satellite cell transplantation is key for studying muscle regeneration. This protocol details isolating and transplanting satellite cells from green fluorescent protein (GFP)-expressing mice to assess regeneration factors.

Area of Science:

Muscle biology and regeneration research
Stem cell transplantation techniques
Endocrinology and metabolic research

Background:

Satellite cells (SCs) are crucial for muscle repair and regeneration.
Investigating SC behavior requires robust experimental models.
Hormonal influences on muscle regeneration are an area of active research.

Purpose of the Study:

To describe a protocol for satellite cell isolation and transplantation.
To utilize this protocol to study the effects of unacylated ghrelin on muscle regeneration.
To evaluate the impact of increased unacylated ghrelin on donor SC engraftment.

Main Methods:

Isolation of satellite cells from green fluorescent protein (GFP)-expressing mouse models.
Transplantation of isolated SCs into recipient murine muscles.

Keywords:

Cardiotoxin Engraftment Muscle excision Muscle stem cells Regeneration Satellite cells Skeletal muscle Transplant

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Last Updated: Oct 31, 2025

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Published on: April 8, 2014

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

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Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

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Assessment of SC engraftment and muscle regeneration parameters.

Main Results:

Successful establishment of a protocol for SC isolation and transplantation.
Demonstration of the protocol's utility in studying muscle regeneration.
Initial findings on the influence of unacylated ghrelin on SC engraftment.

Conclusions:

Satellite cell transplantation is a viable method for studying muscle regeneration.
The described protocol facilitates research into SC biology and regenerative processes.
Further investigation is warranted to fully elucidate the role of unacylated ghrelin in muscle repair.