Related Experiment Video
Updated: Oct 31, 2025

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
Coupling Exonuclease Digestion with Selective Chemical Labeling forBase-resolution Mapping of 5-Hydroxymethylcytosine
Aurélien A Sérandour1,2, Stéphane Avner3, Gilles Salbert3
1Ecole Centrale de Nantes, Nantes, France.
Abstract:
This protocol is designed to obtain base-resolution information on the level of 5-hydroxymethylcytosine (5hmC) in CpGs without the need for bisulfite modification. It relies on (i) the capture of hydroxymethylated sequences by a procedure known as 'selective chemical labeling' (see Szulwach et al., 2012 ) and (ii) the digestion of the captured DNA by exonucleases. After Illumina sequencing of the digested DNA fragments, an ad hoc bioinformatic pipeline extracts the information for further downstream analysis.
Related Concept Videos
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Base Excision Repair
The first step of...

