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Updated: Oct 31, 2025
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
Identification and quantification of cyclitols and sugars isolated from different morphological parts of Raphanus
Hossam Al-Suod1,2, Ileana-Andreea Ratiu2,3, Renata Gadzała-Kopciuch1,2
1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
Abstract:
Through this study, we aimed to develop a new analytical method for identification and quantification of sugars and cyclitols isolated from different morphological parts of Raphanus sativus L (R. sativus). Accelerated solvent extraction with water was involved for targets extraction. Solid phase extraction was used for purification and preconcentration, while high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) was used for chromatographic analyses. A short method of only 30 min for a single analysis was developed finally. The obtained results, allowed for quantification of eight targets, i.e., three cyclitols (D-pinitol, allo-inositol and scyllo-inositol) and five sugars (xylose, D-mannose, D-fructose, D-glucose and sucrose) that were determined simultaneously using a single analysis. The developed method can be applied in industry as a routine method for analysis of sugars and cyclitols from different sources.
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