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Updated: Oct 31, 2025

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
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Imaging the Gut with "CLARITY".

Bindu Chandrasekharan1, Andrew S Neish2

  • 1Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA.

Journal of Visualized Experiments : Jove
|June 28, 2021
PubMed
Summary

CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging compatible Tissue hYdrogel) preserves 3D mouse gut tissue structure for immunostaining. This delipidation method enables detailed cell tracing in gut homeostasis and disease research.

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In gastric emptying studies, a meal's liquid and...
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