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Updated: Oct 31, 2025

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
08:50

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat

Published on: July 3, 2013

23.9K

Hypertension and the Kidney: Reduced Kidney Mass Is Bad for Both Normotensive and Hypertensive Rats

Kyle Ware1, Vedat Yildiz2, Min Xiao1

  • 1Department of Pathology, The Ohio State University, Columbus, Ohio, USA.

American Journal of Hypertension
|June 28, 2021
PubMed
Abstract

Insights

Hypertension exacerbates kidney injury after 5/6 nephrectomy, leading to worse outcomes in hypertensive rats. Histology reveals more severe damage than blood markers alone.

Area of Science:

  • Nephrology
  • Cardiovascular Research
  • Experimental Pathology

Background:

  • Hypertension is a primary driver of chronic kidney disease globally.
  • Previous research focused on short-term hypertension effects on kidney function post-nephrectomy.
  • This study examines the long-term impact of hypertension on kidney injury in a 5/6 nephrectomy model.

Purpose of the Study:

  • To investigate the long-term consequences of hypertension in rats undergoing 5/6 nephrectomy.
  • To compare kidney injury progression in hypertensive versus normotensive rats post-nephrectomy.

Main Methods:

  • Created 5/6 nephrectomy in Spontaneous Hypertensive Rats (SHR) and Wistar Kyoto (WKY) rats.
  • Monitored blood pressure, serum creatinine, hematuria, and proteinuria weekly for 23 weeks.
  • Assessed kidney morphology at study completion; used sham-operated rats as controls.

Main Results:

  • 5/6 nephrectomy increased serum creatinine, blood pressure, hematuria, and proteinuria in both SHR and WKY rats.
  • 5/6 nephrectomy SHR showed earlier onset and higher proteinuria than WKY rats.
  • Histological analysis revealed enlarged glomeruli, interstitial fibrosis, and intimal thickening in 5/6 nephrectomy SHR compared to WKY rats.

Conclusions:

  • Long-term kidney injury severity correlated with elevated blood pressure.
  • Kidney mass reduction worsens kidney function and elevates blood pressure in both normotensive and hypertensive rats.
  • Histological assessment offers superior insight into chronic kidney injury compared to serum creatinine and urinalysis alone.
Keywords:
5/6 nephrectomyblood pressurechronic kidney diseasehypertensionrenal pathologyspontaneous hypertensive rats

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