Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

317
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
317
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

700
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
700
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

257
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
257

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Molecular Heterogeneity of NRAS Variants in Thyroid Nodules.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2026
Same author

Flexible and scalable inference of spatially varying correlation in spatial transcriptomics with spCorr.

Genome research·2026
Same author

IntegrateRigor: annotation-free integration optimization for cell identity recovery reveals cancer-immune interface niches.

bioRxiv : the preprint server for biology·2026
Same author

SnakeAltPromoter Facilitates Differential Alternative Promoter Analysis.

Computational and structural biotechnology journal·2026
Same author

A machine learning approach to predicting dyspnea with noninvasive biomarkers.

Respiratory physiology & neurobiology·2026
Same author

scDesignPop generates realistic population-scale single-cell RNA-seq for power analysis, benchmarking, and privacy protection.

bioRxiv : the preprint server for biology·2026
Same journal

Integrated lipidomic and transcriptomic profiling of the host response in human malaria.

Genome biology·2026
Same journal

Centromeric satellite expansion drives genome evolution in the snowy owl.

Genome biology·2026
Same journal

Mapping the landscape of allele-specific expression in porcine genomes.

Genome biology·2026
Same journal

Genomic sequence evolution underlying human neocortical interareal diversification.

Genome biology·2026
Same journal

Regulatory mechanisms driven by functional 3'-UTR variants in alcohol use disorder and related traits.

Genome biology·2026
Same journal

A longitudinal single-nucleus transcriptomic atlas of bovine placentation reveals dynamic cellular hierarchies and regulatory programs.

Genome biology·2026
See all related articles

Related Experiment Video

Updated: Oct 31, 2025

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.1K

mbImpute: an accurate and robust imputation method for microbiome data.

Ruochen Jiang1, Wei Vivian Li1,2, Jingyi Jessica Li3,4,5,6

  • 1Department of Statistics, University of California, Los Angeles, 90095-1554, CA, USA.

Genome Biology
|June 29, 2021
PubMed
Summary
This summary is machine-generated.

We developed mbImpute, a novel method to accurately identify and correct non-biological zeros in microbiome data. This improves the discovery of disease-associated microbes, ensuring more reliable scientific findings.

More Related Videos

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

39.3K
Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
05:28

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Published on: May 2, 2022

2.3K

Related Experiment Videos

Last Updated: Oct 31, 2025

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.1K
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

39.3K
Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
05:28

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Published on: May 2, 2022

2.3K

Area of Science:

  • Microbiome Research
  • Bioinformatics
  • Computational Biology

Background:

  • Non-biological zeros present a significant challenge in microbiome data analysis.
  • These zeros distort taxon abundance distributions, complicating analysis and impacting scientific discovery.

Purpose of the Study:

  • To introduce mbImpute, the first imputation method designed to address non-biological zeros in microbiome datasets.
  • To enhance the accuracy and reliability of microbiome data analysis.

Main Methods:

  • mbImpute leverages information from similar samples, taxa, and optional metadata (covariates, phylogeny).
  • The method identifies and imputes likely non-biological zero counts.

Main Results:

  • mbImpute significantly improves the power to detect disease-related taxa in type 2 diabetes and colorectal cancer datasets.
  • The method effectively preserves the natural distribution of non-zero taxon abundances.

Conclusions:

  • mbImpute offers a robust solution for handling non-biological zeros in microbiome data.
  • This advancement facilitates more accurate identification of microbial biomarkers for diseases.