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Related Concept Videos

Mass Spectrum01:23

Mass Spectrum

3.0K
A mass spectrum is the graphical representation of the relative abundance of the charged fragments in an analyte plotted against their mass-to-charge ratio (m/z). The plot's x axis represents the ratio of the mass of the charged fragment to the elementary charge it carries. The y axis of the plot represents the relative abundance of each charged species. The relative abundance is calculated from the signal intensity of each charged species recorded at the detector. The most intense signal (the...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
1.9K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

7.1K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Mass Analyzers: Overview01:13

Mass Analyzers: Overview

1.1K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Updated: Oct 31, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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Universal Spectrum Identifier for mass spectra.

Eric W Deutsch1, Yasset Perez-Riverol2, Jeremy Carver3

  • 1Institute for Systems Biology, Seattle, WA, USA. edeutsch@systemsbiology.org.

Nature Methods
|June 29, 2021
PubMed
Summary
This summary is machine-generated.

Mass spectrometry proteomics studies require traceable spectral evidence. The Universal Spectrum Identifier (USI) provides a standardized way to link publication findings to specific mass spectra in public repositories, enhancing data transparency.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Bioinformatics

Background:

  • Mass spectrometry is crucial for proteomics research.
  • Verifying findings requires direct access to supporting mass spectra.
  • Current methods for spectral traceability can be inconsistent.

Purpose of the Study:

  • To introduce and promote the Universal Spectrum Identifier (USI).
  • To standardize spectral identification in public proteomics repositories.
  • To enhance the transparency and reproducibility of proteomics research.

Main Methods:

  • Development of the Universal Spectrum Identifier (USI) standard.
  • Integration of USI into public proteomics data repositories like ProteomeXchange.
  • Encoding virtual paths to mass spectra within datasets.

Main Results:

  • Over 1 billion USI identifications are currently available.
  • These USIs link to more than 3 billion spectra.
  • USI facilitates direct access to spectral evidence for published findings.

Conclusions:

  • The Universal Spectrum Identifier (USI) significantly improves data transparency in proteomics.
  • USI enables robust traceability of conclusions to spectral evidence.
  • Widespread adoption of USI supports the integrity of mass spectrometry-based research.