Electrochemical Column Cell for Continuous Oxidative Inactivation of Pathogens and Reductive Removal of Toxic Heavy...

A solar-driven electrochemical column (EC) was developed for cathodic sequestration remediation of heavy metals (HMs) and anodic electroporative inactivation of pathogenic bacteria (PB) with continuous flow capacity for sustainable production of drinking water from wastewater. The method produces "revitalized drinking water" by keeping its natural mineral nutrients boosted with dissolved oxygen. The EC was constructed with graphene oxide (GO) synthesized via photoassisted electrochemical oxidation of CF (PEGO-CF) as the cathode and phytoreduced GO (RPEGO-CF) as the anode. In the EC, effluent is passed upward through the microchannel of CF electrodes to obtain a higher contact time with water molecules, enabling deposition of HMs and oxidative inactivation of PB, collectively termed electroadsorptive dialysis (EAD). PEGO-CF and RPEGO-CF stacked inside the EC resulted in the increased surface area and thereby the removal efficiency. Reactive oxygen species (ROS) produced at the anode damaged the bacterial cell sheath, while the oxygen functional group and the cathodic negative potential had a concurrent effect in "sequestration" of HMs. Density functional calculations showed that PEGO might transfer an electron from the highest occupied molecular orbital (HOMO) to the lowest unoccupied molecular orbital (LUMO) under applied negative potential leading to internal system crossing to the vacant d-orbitals of HMs, allowing for simultaneous coordination and deposition. The EC produced 313 L of revitalized water from wastewater augmented with 500 μg L^-1 HMs and 10⁷ CFU mL^-1 pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Only a 3.6 J energy investment produced 1 L of revitalized water, which is ∼2000 times less than the usual energy consumption by electroporation and the lowest value obtained to date for bacterial inactivation with heavy metal removal. Laboratory-to-industrial scale-up calculations were performed for this water-purifying technology involving a water-energy nexus, promising high-efficiency bacterial inactivation, and HM remediation to obtain energy-efficient clean and revitalized water.