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Trps1 targets Ccnd1 to regulate mouse Leydig cell proliferation.

Jiandong Sun1, Hua Li1,2, Chengyu Lv1

  • 1Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, P. R. China.

Andrology
|June 29, 2021
PubMed
Summary

Tricho-rhino-phalangeal syndrome-1 gene (Trps1) regulates mouse Leydig cell proliferation by affecting the Src/Akt/Ccnd1 pathway. This study reveals Trps1

Keywords:
Ccnd1CskLeydig cellsSrcin1Trps1

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Area of Science:

  • Molecular and Cellular Biology
  • Reproductive Endocrinology
  • Gene Regulation

Background:

  • Tricho-rhino-phalangeal syndrome-1 gene (Trps1), an atypical GATA family member, is primarily studied in the context of tumors.
  • The role of Trps1 in the male reproductive system, specifically in Leydig cells, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of Trps1 in mouse Leydig cells.
  • To elucidate the regulatory mechanism of Trps1 on Leydig cell cycle progression.

Main Methods:

  • Utilized gene-silencing technology (RNA interference) in primary mouse Leydig cells and MLTC-1 Leydig cell line.
  • Employed RNA-sequencing (RNA-seq), RT-qPCR, and western blotting to assess gene and protein expression.
  • Conducted chromatin immunoprecipitation (ChIP) assays (ChIP-seq and ChIP-qPCR) to determine Trps1's regulatory mechanism.

Main Results:

  • Knockdown of Trps1 significantly suppressed Src and Akt phosphorylation and Ccnd1 expression, impairing Leydig cell proliferation.
  • Trps1 appears to influence the cell cycle via the Src/Akt/Ccnd1 signaling pathway.
  • Trps1 may directly bind to the Srcin1 promoter, regulating its transcription and subsequently affecting Src phosphorylation and Leydig cell proliferation.

Conclusions:

  • Trps1 plays a crucial role in regulating mouse Leydig cell proliferation.
  • The Trps1-mediated regulation involves the Src/Akt/Ccnd1 signaling pathway, potentially through modulating Srcin1 expression.
  • These findings provide novel insights into the function of Trps1 in male reproductive cell proliferation and differentiation.