Calibration of an in-situ fluorescence-based sensor platform for reliable BOD₅ measurement in wastewater

Reliance on biochemical oxygen demand (BOD₅) as an indicator of wastewater quality has hindered the development of efficient process control due to the associated uncertainty and lag-times. Surrogate measurements have been proposed, with fluorescence spectroscopy a promising technique. Yet, assessment of in-situ fluorescence sensors across multiple wastewater treatment plants (WwTPs), and at different treatment stages, is limited. In this study a multi-parameter sonde (two fluorescence peaks, turbidity, temperature and electrical conductivity) was used to provide a BOD₅ surrogate measurement. The sonde was deployed at three WwTPs, on post primary settlement tanks (PST) and final effluent (FE). Triplicate laboratory measurements of BOD₅, from independent laboratories were used to calibrate the sensor, with high variability apparent for FE samples. Site and process specific sensor calibrations yielded the best results (R²_cv = 0.76-0.86; 10-fold cross-validation) and mean BOD₅ of the three laboratory measurements improved FE calibration. When combining PST sites a reasonable calibration was still achieved (R²_cv = 0.67) suggesting transfer of sensors between WwTPs may be possible. This study highlights the potential to use online optical sensors as robust BOD₅ surrogates in WwTPs. However, careful calibration (i.e. replicated BOD₅ measurements) is required for FE as laboratory measurements can be associated with high uncertainty.